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Applications of Biotechnology

The plasmid c...

Question

The plasmid called as recombinant plasmid and used for introduction of large genome to host cells is?

A

Lamda

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B

2μ plasmid

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C

Cosmid

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D

Fertility factor

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Solution

The correct option is D Cosmid
Cosmids are cloning vectors that were developed to enable large fragments of DNA to be cloned and maintained.
Cosmid vectors allow the cloning of fragments up to 45 kilobases (kb) and are commonly used in genomic library construction.
Cosmid vectors are plasmids with a small region of cos sequence of bacteriophage.

Thus, the correct answer is option C.

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Q. The plasmid called recombinant plasmid and used for introduction of large genome to host cells i ___________.

Q. The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps ?

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. Read the given statements and select the correct option.
Statement 1: The tumour inducing plasmid (Ti plasmid) acts as a cloning vector in recombinant DNA technology.
Statements : The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.

Q. Explain any two methods for introduction of recombinant DNA into host cell.

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