Question

The plasmid derived from $E.coli$ is

• A. pBR327
• B. pBR322
• C. Both (A) and (B)
• D. None of the above

Answer 1

The correct option is C Both (A) and (B)

Simplest cloning vectors based on small bacterial plasmids are the most widespread in gene cloning experiment. A large number of different plasmid vectors are available for use with E. coli and these vectors combine ease of purification with desirable properties such as high transformation efficiency, convenient selectable markers for transformants and recombinants, and the ability to clone reasonably large pieces of DNA. One of the most popular vector used in gene cloning is pBR322. pBR327 plasmid is a higher copy number plasmid and was constructed by removing a 1089 bp segment from pBR322. Thus, the correct answer is option C.