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Question

The polymerase chain reaction is a technique that

A
Is used for in vivo replication of DNA
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B
Is used for in vivo synthesis of mRNA
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C
Is used for in vitro synthesis of rRNA
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D
Is used for in vitro amplification of specific DNA sequence using thermostable DNA polymerase
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Solution

The correct option is D Is used for in vitro amplification of specific DNA sequence using thermostable DNA polymerase
Polymerase chain reaction (PCR) was discovered by Kary Mullis. It is a technique by which DNA can be cloned or amplified in vitro by a thermostable enzyme DNA Taq Polymerase. PCR has three steps: Denaturation, Annealing and Extension.
So, the correct answer is 'Is used for in vitro amplification of specific DNA sequence using thermostable DNA polymerase'

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