Question

The polymerase chain reaction is a technique that

• A. Is used for in vivo replication of DNA
• B. Is used for in vivo synthesis of mRNA
• C. Is used for in vitro synthesis of rRNA
• D. Is used for in vitro amplification of specific DNA sequence using thermostable DNA polymerase

Answer 1

The correct option is D Is used for in vitro amplification of specific DNA sequence using thermostable DNA polymerase

Polymerase chain reaction (PCR) was discovered by Kary Mullis. It is a technique by which DNA can be cloned or amplified in vitro by a thermostable enzyme DNA Taq Polymerase. PCR has three steps: Denaturation, Annealing and Extension.

So, the correct answer is 'Is used for in vitro amplification of specific DNA sequence using thermostable DNA polymerase'