The correct option is D In situ conservation
Recalcitrant seeds (subsequently known as unorthodox seeds) are seeds that do not survive drying and freezing during ex-situ conservation and visa versa. Conservation refers to protection of genetic diversity of crop plants from genetic erosion. There are two important methods of germplasm conservation or preservation- 1) in situ conservation and 2) ex situ conservation.
Conservation of germplasm under natural habitat is referred to as in situ conservation. It requires establishment of natural or biosphere reserved national parks or protection of endangered areas or species. In this method of conservation, the wild species and the complete natural or semi natural ecosystem are preserved together. This method of preservation has following main disadvantages.
1. Each protected are will cover only very small portion of total diversity of a crop species, hence several areas will have to be conserved for a single species.
2. The management of such areas also poses several problems.
3. This is a costly method of germplasm conservation.