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"The presence...

Question

"The presence of chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert .presence of insert results into insertional inactivation of Beta galactosidase and recombinant colonies do not produce any colour these are identified as Recombinant Colony" explain these lines.

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Solution

Dear student,
Non recombinant bacteria forms blue colonies due to the activation of the enzyme alpha galactosidase which hydrolyses alpha glyctosyl moiety from glycolipid and glycoproteins. But when recombinant bacteria is formed, the gene (GLA) encoding alpha galactosidase enzyme becomes inactivated due to insertion so white colony of bacteria is formed.

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Q. During insertional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the enzyme

Q. Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement 2: Presence of insert results into insertional inactivation of -galactosidase enzyme and the colonies do not produce any colour.

Q. Read the given statements and select the correct option.
Statement-1: In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement-2: Presence of insert results into insertional inactivation of beta- galactosidase enzyme and the colonies do not produce any colour.

Q. Assertion: : Insertion of recombinant DNA within the coding sequence of beta-galactosidase results in colourless colonies.

Reason: Presence of insert results in inactivation of enzyme beta-galactosidase known as insertional inactivation.

Q. After insertion of DNA segment within the sequence of Z-gene of bacteria, they are grown on chromogenic substrate. After the growth of bacterial colonies, they are identified as
i) Recombinants if colonies are blue- coloured.
ii) Recombinants if colonies are white coloured.
iii) Non-recombinants if colonies are blue- coloured.
iv) Non-recombinants if colonies are white coloured.

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