The problem of necrosis and senescence in tissue culture is overcome by

Spraying cytokinins

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Spraying auxins

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Suspension culture

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Subculture

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Solution

The correct option is D Subculture
D. Subculture. Solution: Necrosis and gradual senescence are caused due to faster depletion of food and ethylene production in culture. As the biomass grow their is depletion of food, and increase in the growth regulators ethylene. Some plants are sensitive to ethylene, so it is suggested to subculture before necrosis occurs.

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