(b) elemental form of oxygen
Certain bacteria such as Azotobacter (occur freely in the soil) and Fihizobium (occur in root nodules of leguminous plants), convert the atmospheric nitrogen into water soluble nitrates. The process of bio fixation of nitrogen is called as nitrogen fixation.
Enzymes required for nitrogen fixation are nitrogenases. These nitrogenases are very susceptible to destruction by oxygen. Many bacteria cease the production of enzymes in the presence of oxygen. Many nitrogen fixing bacteria grow in anaerobic conditions or respire to bring down oxygen levels.