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Question
The process of transformation is affected by which of the following enzymes?
The process of transformation is affected by which of the following enzymes?
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Solution
The correct option is A DNAase
Oswald Avery, Colin MacLeod and Maclyn McCarty (1933-44) purified biochemicals (proteins, DNA, RNA, etc.) from the heat-killed S cells to see which ones could transform live R cells into S cells. They discovered that DNA from S bacteria caused R bacteria to become virulent. They also discovered that protein-digesting enzymes (Peptidase) and RNA-digesting enzymes (RNases) did not affect transformation, so the transforming substance was not a protein or RNA. Digestion with DNase did inhibit transformation, suggesting that the DNA caused the transformation. Thus they concluded that DNA is the hereditary material,
Oswald Avery, Colin MacLeod and Maclyn McCarty (1933-44) purified biochemicals (proteins, DNA, RNA, etc.) from the heat-killed S cells to see which ones could transform live R cells into S cells. They discovered that DNA from S bacteria caused R bacteria to become virulent. They also discovered that protein-digesting enzymes (Peptidase) and RNA-digesting enzymes (RNases) did not affect transformation, so the transforming substance was not a protein or RNA. Digestion with DNase did inhibit transformation, suggesting that the DNA caused the transformation. Thus they concluded that DNA is the hereditary material,
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