The reaction which is responsible for the primary fixation of CO2 is catalysed by
RuBP carboxylase and PEP carboxylase
RuBP carboxylase is responsible for the fixation of CO2 in C3 plants, while PEP carboxylase fixes CO2 in C4 plants. RuBP carboxylase uses ribulose-1, 5- bisphosphate as the CO2 acceptor for the carboxylation reaction, while PEP carboxylase uses phosphoenol pyruvate as a CO2 acceptor for carboxylation.
RuBisCO is the most abundant enzyme in the world and present in very high concentration in chloroplasts. It is required in very high concentration for