Question

The recent techniques used for separating fragments of DNA is

• A. Northern blotting
• B. Southern blotting
• C. Eastern blotting
• D. Western blotting

Answer 1

The correct option is B Southern blotting

Southern blotting is a common technique used in molecular biology to separate and characterize DNA. Southern blotting is a technique used to determine the presence of a specific DNA sequence within a mixture via agarose gel electrophoresis. The Northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. The Eastern blot is a biochemical technique used to analyze protein post translational modifications such as lipids, phospho-moieties and glycoconjugates. Western blotting is an adaptation of the Southern blot procedure, used to identify specific amino-acid sequences in proteins.