Question

The reduction in the enzyme activity takes place by
I. Decreasing pH level
II. Increasing temperature
III. Placing enzyme in a non polar liquid

• A. I only
• B. II only
• C. III only
• D. I and II
• E. I, II, and III

Answer 1

Answer: (E)

I, II, and III

Enzyme activity is a measure of how many enzymes are present in the reaction. Enzyme activity can be affected by a number of factors which include pH, temperature, concentration of enzyme and substrate etc. Increase in temperature decrease in the level of pH reduces the enzyme activity as above the optimum temperature and below the optimum pH the enzyme structure begins to break down (denature) since at higher temperatures Intra- and intermolecular bonds are broken as the enzyme molecules gain even more kinetic energy.

Also, because proteins function in an aqueous (water) solution and water is polar, a protein will fold with its hydrophilic (water seeking) amino acids closer to the surface and its hydrophobic (water-avoiding) amino acids closer to the center. Moving the enzyme to a nonpolar solution will alter its tertiary structure, as hydrophobic amino acids move to the surface instead.

Therefore, the correct answer is option E.