The correct option is C RNA polymerase III
RNA polymerase is an enzyme that catalyses the formation of the RNA strand using DNA as the template during transcription.
In eukaryotes, there are three different types of RNA polymerases.
RNA polymerase I- transcribes rRNA (28S, 18S and 5.8S).
RNA polymerase II- responsible for the transcription of the precursor of mRNA, the heterogeneous nuclear RNA (hnRNA).
RNA polymerase III- responsible for the transcription of tRNA, 5srRNA and snRNA (small nuclear RNA).
Thus, the removal of RNA polymerase III will affect the synthesis of 5srRNA.
Helicases are enzymes that use ATP to break the hydrogen bonds in the DNA. This action helps access the genetic information on the DNA for replication.