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Complement of an Event

The restricti...

Question

The restriction cut by enzyme BamH I that recognizes a palindromic sequence and leaves one strand longer than the other would produce a DNA that looks like

A

. . . G A T C C - 3^{'} . . . C - 5^{'}

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B

. . . C - 3^{'} . . . G A T C C - 5^{'}

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C

. . . G - 3^{'} . . . C - 5^{'}

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D

. . . G - 3^{'} . . . C C T A G - 5^{'}

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E

. . . C T A G G - 3^{'} . . . G A T C C - 5^{'}

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Solution

The correct option is D $. . . G - 3^{'} . . . C C T A G - 5^{'}$
The restriction site for BamHi is (5′–G/GATCC–3′). It makes a staggered cut and leaves “sticky” ends having G-3' and -CCTAG-5' overhang. Thus, the correct answer is option D.

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Q. A student wants to construct a plasmid 'BacLight', that when transformed into a bacterium will produce light IF AND ONLY IF the sugar lactose is present in the medium. For this, she uses the following DNA sequences: promoter of repressor

(P_{i})

, promoter of lacZ

(P_{z})

, operator (O) and repressor gene lacI from the lactose operon; and a structural gene X, which encodes a protein that produces light. The different DNA elements (II-V) and a vector (I) were digested with restricted enzymes and ligated to produce the plasmid. The DNAs used along with restriction enzyme sites at their ends are given. Slash(/) in the sequence represents the point at which the restriction enzyme cuts the DNA.
I:

5^{'}

-(G/GATCC)-Vector-(T/CTAGA)-

3^{'}

5^{'}

P_{i}

-lacI-(G/GATCC)-

3^{'}

5^{'}

P_{z}

3^{'}

5^{'}

-(G/CTAGC)-O-(A/CTAGT)-

3^{'}

5^{'}

-(A/CTAGT)-Gene X-(T/CTAGA)-

3^{'}

Choose the correct sequence of DNAs that can possibly be ligated to produce the plasmid 'BacLight'.

Q. (a) Write the palindromic nucleotide for the following DNA segment:
5-G AA TTC-3
(b)Name the restriction endonuclease that recognises the sequence

(C)How are sticky-ends produced ? Mention their role.

Q. The complementary strand for the following single strand of DNA is:

5^{'} \leftarrow A - T - G - C - T \to 3^{'}

Q. The restriction endonuelease EcoR-1 recognises and cleaves DNA sequence as shown below
5' - G A A T T C - 3'
3' - C T T A A G - 5'
What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence ?

Q. The restriction enzyme responsible for the cleavage of following sequence is
5' - G - T - C - G - A - C - 3'
3' - C - A - G - C - T - G - 5'

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