Question

The restriction enzyme responsible for the cleavage of following sequence is
5' - G - T - C - G - A - C - 3'
3' - C - A - G - C - T - G - 5'

• A. Alu I
• B. Bam HI
• C. Hind II
• D. Eco RI

Answer 1

The correct option is A Alu I

• A restriction enzyme is an enzyme that cleaves the DNA molecule at a specific site called a restriction site. It is used to cut DNA into smaller fragments.
• Different restriction enzymes have different restrictions sites and hence cut different yet specific DNA sequences. There are two types of restriction enzymes - endonucleases and exonucleases.
• Exonucleases cut DNA from the end while the endonucleases cut the DNA by recognizing a specific sequence of base pairs. Alu I is an exonucleases restriction enzyme that recognizes and cuts at the AG^CT site.

Thus, the correct answer is 'Alu I.'