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Blunt and Sticky Ends

The restricti...

Question

The restriction enzyme(s) used in recombinant DNA technology making staggered cuts in DNA leaving sticky ends is/are

A

Eco RI

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B

Hind III

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C

Bam HI

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D

All of these

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Solution

The correct option is D All of these
In a staggered cut or sticky end cut, the cuts are made in two strands of DNA at two different sites few nucleotides away from each other, thus producing two strands with protruding ends. Enzymes Eco RI, Hind III, Bam HI, Bgl II, Pst I, etc., produce this type of ends.

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Q. The restriction enzymes used in recombinant DNA technology that make staggered cuts in DNAleaving sticky ends is/are

Q. Assertion: Sticky ends forms hydrogen bonds with their complementary cut counterparts.
Reason: In recombinant DNA technology, sticky end producing restriction enzymes are preferred over blunt ends producing restriction enzymes.

Q. Which type of restriction enzymes are used in recombinant DNA technology?

Q. Arrange the following steps of formation of recombinant DNA in the correct sequence.
I. Complementary sticky ends are produced on the foreign DNA and the plasmid.
II. Foreign DNA and plasmids are mixed together
III. DNA ligase is used to seal the nicks in the sugar–phosphate backbone.
IV. Cut the foreign DNA (containing the DNA fragment of interest) and the plasmid with the same restriction enzyme.

Q. Assertion :Restriction enzymes cut the strand of DNA to produce sticky ends. Reason: Stickiness of the ends facilities the action of the enzyme DNA polymerase.

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