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Question

The restriction enzymes used in recombinant DNA technology that make staggered cuts in DNA leaving sticky ends is/are

A
Eco RI
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B
Hind III
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C
Bam HI
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D
All of these
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Solution

The correct option is D All of these
There are over 500 restriction endonuclease that have been isolated till date. Some of them cut the DNA leaving blunt ends, some of them cut the DNA leaving sticky ends. Eco RI, Hind III and Bam HI are restriction endonucleases that leave a sticky end when they cut the DNA at their recongnition site.

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