The role of DNA ligase in the construction of a recombination DNA molecule is:

Formation of phosphodiester bond between two DNA fragments

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Formation of hydrogen bonds between sticky ends of DNA fragments

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Ligation of all purine and pyrimidine bases

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Formation of hydrogen bonds between blunt ends

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Solution

The correct option is A Formation of phosphodiester bond between two DNA fragments
DNA ligase (joining or sealing enzymes) join two individual fragments of double stranded DNA by forming phosphodiester bonds between them. Thus, they help in sealing gaps in DNA fragments. Therefore, they act as a molecular glue.

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