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Question

The sequence complementary to the sequence of the spliced and mature mRNA is .

A
rDNA
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B
cDNA
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C
genomic DNA
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D
protein sequence
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Solution

The correct option is B cDNA
cDNA or complementary DNA is formed from mature mRNA using reverse transcriptase enzyme. The gene for a protein in genomic DNA has both coding and noncoding regions called as exons and introns respectively. If this DNA is used in this form for genetic transformation noncoding regions will also produce amino acids which can change the original protein. To avoid such complications genetic technologists make use of reverse transcriptase to make DNA from mature mRNA which has only coding sequences and no introns.

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