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Question
The sequence that controls the copy number of the linked DNA in the vector, is termed
The sequence that controls the copy number of the linked DNA in the vector, is termed
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Solution
The correct option is D ori site
Vectors are used to carry the gene of interest in biotechnology.
ori is an abbreviation for the origin of replication. It is a sequence of DNA in the vector from where replication starts. It helps in replication of vectors independent of the genomic DNA when inserted into host cells. This sequence is also responsible for controlling the copy number (number of copies per host cell) of the linked DNA. Therefore, ori is essential to make copies of the vector in the host cell.
The specific sequences which are recognized by the restriction enzymes are called recognition sequences or recognition sites. Recognition sequences are generally palindromic.
Palindromic sequences are sequences of base pairs in the DNA that reads the same on both the strands when the orientation of reading is kept the same.
Selectable markers are important in a vector. They help in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
Selectable markers are often antibiotic resistance genes.
Vectors are used to carry the gene of interest in biotechnology.
ori is an abbreviation for the origin of replication. It is a sequence of DNA in the vector from where replication starts. It helps in replication of vectors independent of the genomic DNA when inserted into host cells. This sequence is also responsible for controlling the copy number (number of copies per host cell) of the linked DNA. Therefore, ori is essential to make copies of the vector in the host cell.
The specific sequences which are recognized by the restriction enzymes are called recognition sequences or recognition sites. Recognition sequences are generally palindromic.
Palindromic sequences are sequences of base pairs in the DNA that reads the same on both the strands when the orientation of reading is kept the same.
Selectable markers are important in a vector. They help in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
Selectable markers are often antibiotic resistance genes.
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