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Question
The specific DNA sequence where EcoRl cuts is
The specific DNA sequence where EcoRl cuts is
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Solution
The correct option is B GAATTC
CTTAAG
Restriction enzymes like EcoRI, cut through the DNA strands at nucleotides that are not exactly opposite each other. This creates DNA fragments with one nucleotide strand that overhangs at the end. This overhanging nucleotide strand is called as a sticky end because it can easily bond with complementary DNA fragments. It creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry.Thus, the correct answer is option B.
CTTAAG
Restriction enzymes like EcoRI, cut through the DNA strands at nucleotides that are not exactly opposite each other. This creates DNA fragments with one nucleotide strand that overhangs at the end. This overhanging nucleotide strand is called as a sticky end because it can easily bond with complementary DNA fragments. It creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid sequence where the enzyme cuts is GAATTC, which, as the complementary sequence is CTTAAG, has rotational symmetry.
Thus, the correct answer is option B.
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