254
Question
The stain used by Feulgen to stain DNA is
The stain used by Feulgen to stain DNA is
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Solution
The correct option is A Basic fuchsin
The Feulgen reaction is a technique developed by Feulgen and Rossenbeck and it is the standard technique for demonstrating deoxyribose. Mild acid hydrolysis (1M HCL, at 60oC) is used to break the purine-deoxyribose bond. The resulting aldehydes are then demonstrated by the use of Schiff's reagent. Schiff's reagent is formed by the reaction of sulfur dioxide with the dye basic fuchsin to produce a colorless compound known as leuco-basic fuchsin which reacts with exposed aldehydes and DNA is stained red-purple color. RNA does not demonstrate this reaction.Thus, the correct answer is option B.
The Feulgen reaction is a technique developed by Feulgen and Rossenbeck and it is the standard technique for demonstrating deoxyribose. Mild acid hydrolysis (1M HCL, at 60oC) is used to break the purine-deoxyribose bond. The resulting aldehydes are then demonstrated by the use of Schiff's reagent. Schiff's reagent is formed by the reaction of sulfur dioxide with the dye basic fuchsin to produce a colorless compound known as leuco-basic fuchsin which reacts with exposed aldehydes and DNA is stained red-purple color. RNA does not demonstrate this reaction.
Thus, the correct answer is option B.
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