Question

The steps involved in making a slide of epidermal peel of leaf are given as follows :
I. Pull out a thin peel from the lower surface of the leaf
II. Place a drop of glycerine on the slide
III. Stain the peel in safranin
IV. Place the stained peel on the glycerine
V. Remove the extra stain by washing with water
VI. Place the coverslip over the peel
Which one is the correct sequence of steps to be followed?
(1) I, II, III, IV, V, VI
(2) I, III, V, II, IV, VI
(3) I, III, IV, II, V, VI
(4) I, II, IV, III,V, VI

Answer 1

The correct sequence of steps for making a slide of epidermal peel are -
I. Pull out a thin peel from the lower surface of the leaf
III. Stain the peel in safranin
V. Remove the extra stain by washing with water
II. Place a drop of glycerine on the slide
IV. Place the stained peel on the glycerine
VI. Place the coverslip over the peel

I, III, V, II, IV, VI

Hence, the correct answer is option 2.