Question

The subcellular components can be separated by

• A. Paper chromatography
• B. Autoradiography
• C. Gel electrophoresis
• D. Differential and density gradient centrifugation

Answer 1

The correct option is D Differential and density gradient centrifugation

• The subcellular components of eukaryotic cells include, most importantly, the nucleus with its associated nucleolus and all the extranuclear, or 'cytoplasmic', components, which include: ribosomes, endoplasmic reticulum, Golgi apparatus, cytoskeleton, mitochondria, vacuoles, and vesicles.
• These subcellular components can be separated by differential and density gradient centrifugation. The main difference between differential and density gradient centrifugation is that fractionation is carried out based on the size in differential centrifugation whereas fractionation is carried out based on the density in density gradient centrifugation.
• So, the correct answer is 'Differential and density gradient centrifugation'.