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Question

The technique based on a violation of Mendels law of independent assortment is

A
Shotgun genome sequencing
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B
RFLP
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C
PCR
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D
Plasmid cloning and selection
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E
Genome marker mapping
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Solution

The correct option is D Genome marker mapping
According to Mendel's law of independent assortment, during the inheritance of two or more characters, the assortment of individual traits takes place independently during gamete formation. If the genes are located on the same chromosomes, they are called as linked genes. The linkage is strictly against the law of independent assortment where Mendel explains that the characters are sorted out independently while forming gametes. During genetic mapping or the genome marker mapping, we trace for the linked genes or the overlapping fragments. This is the method which works on the principle against this law.
Thus, the correct answer is option E.

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