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Question

The technique in which a foreign DNA is precipitated on the surface of the tungsten or gold particle and shot into target cells is known as?

A
Microinjection
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B
Chemical - mediated genetic transformation
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C
Electroporation
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D
Biolistic method
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Solution

The correct option is D Biolistic method
  • In microinjection, a glass-made micropipette is used for DNA transfer.
  • The chemical-mediated genetic transformation uses chemical agents.
  • In Electroporation, the target cells are mixed and treated with an electric field to create pores for DNA entry.
  • In the biolistic method or gene gun method, the foreign DNA is precipitated over the surface of metals like; gold or tungsten, and bullets are prepared.
  • These bullets are then fired into the solution of target cells. This foreign DNA gets inserted and helps in the production of desired products.
So, the correct option is option D.

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