The correct option is D ICSI
The abbreviations mentioned in the options represent various techniques that can be used to counteract the condition of infertility in males and females. These are collectively referred to as assisted reproductive technologies. Each abbreviation mentioned refers to a unique set of procedures followed to overcome the obstructions in conception.
The technique in which the zona pellucida layer of an oocyte is penetrated to facilitate the artificial insertion of the spermatozoa is referred to as ICSI. ICSI stands for Intracytoplasmic sperm injection. In this technique, a single spermatozoon or even a spermatid is injected directly along with a solution into the cytoplasm of the oocyte using a thin needle. The plasma membrane of the oocyte is referred to as oolemma. Outer to the oolemma lies the zona pellucida layer. To be able to insert the spermatozoon directly into the ooplasm (cytoplasm of the oocyte), the zona pellucida layer has to be penetrated. This is made possible due to ICSI.
ZIFT refers to zygote intra fallopian transfer. In this technique, the zygote that is formed by the in vitro fertilisation of the male and female gametes, is transferred to the fallopian tube of the female for further development.
Cryopreservation refers to the process in which gametes are stored at -196 degree Celsius in liquid nitrogen.
TVOR stands for transvaginal oocyte retrieval. This technique is used for the retrieval of the oocyte from the female parent or willing oocyte donor. A thin needle, guided by sonogram, is inserted through the vaginal wall into the ovaries to extract mature oocyte.