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Question

The technique of gel electrophoresis was developed by

A
Arne Tiselius
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B
Kary Mullis
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C
Nirenberg
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D
Alec Jeffreys
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Solution

The correct option is A Arne Tiselius
Arne Tiselius, a Swedish chemist, developed and introduced the technique of gel electrophoresis as an analytical technique in the 1930s. The technique is based on the principle of movement of charged particles towards oppositely charged electrodes under the influence of the electric field. The DNA fragments of varying sizes experience different forces due to the presence of the electric field. Besides, they also encounter different resistance to move in the gel matrix depending on their size. These factors, altogether, monitor the rate of movement of DNA molecules of varying sizes. Hence, the DNA molecules of different sizes get separated while they migrate towards the oppositely charged electrode (anode) in the presence of an electric field.


Kary Mullis was an American biochemist, who is renowned for his contribution in the invention of polymerase chain reaction, a technique used for DNA amplification.

Marshall Warren Nirenberg was an American biochemist and geneticist who is credited with the cracking of genetic code. This was a breakthrough in deciphering the protein synthesized from the triplet codon.

Sir Alec John Jeffreys is a British geneticist. He is renowned for his enormous contribution in developing the technique of DNA fingerprinting which is widely used in forensic science.

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