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Question

The technique used to increase the amount of DNA available for analysis is
I. Cloning a plasmid
II. Gel electrophoresis
III. PCR

A
II only
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B
I and II
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C
I and III
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D
II and III
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E
I, II, and III
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Solution

The correct option is C I and III
The polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy of DNA to millions of copies of a particular DNA sequence.
Molecular cloning is a method used to assemble recombinant DNA molecules and to direct their replication within host organisms to increase the number of recombinant DNA molecule. Here, the DNA can be cloned in a plasmid (extracellular DNA).
Thus, the correct answer is option C.


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