Question

The technique used to sterilize nutrient media is

• A. Laminar airflow
• B. Hot air oven
• C. Autoclave
• D. Centrifugation

Answer 1

Answer: (C)

Autoclave

Lamina airflow is a device that is used to remove air-born contaminants. It has special filters known as HEPA.

Hot air ovens are electrical devices that use dry heat to sterilize.

An autoclave is a pressure chamber (like a pressure cooker) used to sterilize nutrient media subjecting it to high-pressure of saturated steam at 121 ${}^o$C for around 15–20 minutes that kills all unwanted contaminant microbes.

Centrifugation is a technique that involves the application of centrifugal force to separate particles from a solution according to their size, shape, density, viscosity of the medium, and rotor speed.

So, the correct answer is option C.