The temporary mount of leaf is stained with safranine and mounted with glycerine.
Safranine is a dye which can be taken up by cells and gives them a pink colour. The cell as well as the background are both transparent and it is difficult to visualise the cells as such. Staining imparts colour to the cells or its components and enhances its contrast and makes it easier to see them under the microscope.
Hence, the correct answer is option 3.