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Question

The thermostable enzymes ‘Taq’ and ‘Pfu’, isolated from thermophilic bacteria are

A
DNA ligases
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B
RNA polymerases
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C
Restriction endonucleases
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D
DNA polymerases
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Solution

The correct option is D DNA polymerases
*DNA polymerases are the enzymes that can add deoxyribonucleotides to an already existing DNA strand or primer.

*Hence DNA polymerases are used for in vitro DNA synthesis.

*The thermostable property of thermostable DNA polymerases makes it possible to remain active during the high temperature conditions of PCR, especially in the denaturation of double stranded DNA.

*Examples include ‘Taq and Pfu’ isolated from thermophilic bacteria.

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