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Question

The whole cycle of gene amplification requires

A
15 minutes
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B
30 minutes
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C
3 hours
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D
24 hours.
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Solution

The correct option is B 30 minutes
The denaturation stage of PCR takes about 1–2 minutes. The thermocycler then lowers the temperature to about 50° to 60°C, which allows the short, oligonucleotide primers to anneal to their complementary sequences on the single-stranded DNA molecules. The annealing stage of PCR lasts about 30 seconds. Hence, the whole cycle of gene amplification requires 30 minutes.
So, the correct answer is '30 minutes.'

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