There are several proteins that help to open up DNA double helix in front of the replication fork to stretch supercoils these belong to the category of

DNA topoisomerase

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DNA polymerase I

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DNA gyrase or ligase

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DNA unwindase or DNA ligase

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Solution

The correct option is A DNA topoisomerase
Several enzymes other than the DNA polymerase, participates in the process of DNA replication. DNA topoisomerase associates with parental DNA ahead of the unwinding helicase enzyme to remove torsional stress introduced by the unwinding of the parental strands. DNA gyrase enzyme relives the strain introduced in DNA due to unwinding by helicase. DNA ligase enzyme joins two pieces of DNA.
So, the correct answer is option A.

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Similar questions

Q. Protein helping in opening of DNA double helix in front of replication fork is
a. DNA gyrase
b. DNA polymerase 1
c. DNA Ligase
d. DNA topoisomerase

Q. How many of the following statements are correct ?

A. DNA dependent DNA polymerase requires DNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

B. DNA dependent DNA polymerase requires RNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

C. During replication, unwinding of DNA double helix forms a fork-like structure called replication fork.

D. During transcription, unwinding of the DNA double helix by DNA dependent RNA polymerase forms a transcription bubble.

E. During transcription, unwinding of DNA double helix by DNA dependent RNA polymerase forms a transcription fork.

Match the following enzymes of DNA replication with their respective functions:
$\begin{matrix} p . D N A p o l y m e r a s e & i . H e l p s u n z i p t h e D N A d o u b l e h e l i x q . D N A l i g a s e & i i . S e a l s n i c k s b e t w e e n O k a z a k i f r a g m e n t s r . D N A h e l i c a s e & i i i . L a y s R N A p r i m e r s t o w h i c h p o l y m e r a s e c a n a d d n u c l e o t i d e s s . P r i m a s e & i v . A d d s n u c l e o t i d e s t o t h e f r e e 3^{'} e n d o f R N A p r i m e r s \end{matrix}$

Q. Match List I(Enzyme) with List II(Function) and select the correct answer using the codes given the lists.

List-I	List-II
a. DNA Ligase	$1$ . Catalyses the covalent union of segments of an interrupted sugarphosphate strand in double-stranded DNA
b. DNA polymerase	$2$ . End nuclease that digests either single stranded or double stranded DNA
c. DNase I	$3$ . Produces single or double stranded breaks in DNA during replication to release tension brought about by the separation of double strands
d. DNA topoisomerase	$4$ . Catalyses the formation of DNA worn deoxyribonucleotides using one strand of DNA as template

Q. Explain the mechanism of DNA replication with the help of a replication fork. What role does the enzyme DNA-ligase play in a DNA replication fork?

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