Question

Thin layer chromatography can be used to distinguish between different amino acids. If a particular amino acid has low solubility in the mobile phase used, then the other amino acid :

• A. will have a low Rf value.
• B. must have a high molecular mass.
• C. will move at a speed close to that of the solvent
• D. will spend more time dissolved in the mobile phase than attached to the stationary phase.

Answer 1

The correct option is A will have a low Rf value.

Will have low $R_f$ value.

$R_f$ = $\frac{\text{Distance moved by component}}{\text{Distance moved by solvent front}}$

So, If amino acid has low solubility, the can not move up in mobile phase.