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Question

This method of finding a gene is used when researchers know very little about the gene they are trying to find. This process results in a complete gene library: a collection of copies of DNA fragments that represent the entire genome of an organism. Identify the method.
  1. Cloning
  2. Shotgun cloning
  3. Gene synthesis cloning
  4. PCR


Solution

The correct option is B Shotgun cloning
Shotgun cloning involves cutting the DNA of the entire genome into pieces with restriction enzymes, inserting these pieces or fragments into bacteria or yeast with plasmids or viruses and allowing the organisms to reproduce making copies or clones of the DNA fragments.
DNA cloning is a molecular biology technique that makes many identical copies of a piece of DNA, such as a gene.
Artificial gene cloning is a method of synthetic biology that is used to create artificial genes in the laboratory. It differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. 
Polymerase chain reaction (PCR) is a common laboratory technique used to make many copies (millions or billions!) of a particular region of DNA.

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