Question

This method of finding a gene is used when researchers know very little about the gene they are trying to find. This process results in a complete gene library: a collection of copies of DNA fragments that represent the entire genome of an organism. This process is called as

• A. RFLP
• B. Shotgun cloning
• C. Gene synthesis cloning
• D. PCR

Answer 1

The correct option is B Shotgun cloning

Shotgun cloning is a technique that involves sequencing of gene when only little information is available about the gene. In this, entire DNA is cut into numerous fragments either using restriction enzymes or by physical method. These DNA fragments are then sequenced individually by inserting these fragments into bacteria or yeast with plasmids. It has been used to sequence the mouse, fly and human genomes. Hence, it results in a complete gene library.

Thus, the correct answer is option B.