Question

To carry out a polymerase chain reaction (PCR), one must have the catalytic DNA polymerase and

• A. a blueprint or gene map of the sequence one wish to copy
• B. a number of primers from either side of the target DNA in order to get the polymerase replication process- going
• C. a DNA synthesizer machine
• D. a DNA probe

Answer 1

The correct option is B a number of primers from either side of the target DNA in order to get the polymerase replication process- going

Polymerase Chain Reaction is a method widely used to make many copies of a specific DNA segment. Using PCR, a single (or more) copy of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of that particular DNA segment. Components of PCR include DNA template, DNA polymerase, two DNA primers, nucleotides.

So, the correct option is 'a number of primers from either side of the target DNA in order to get the polymerase replication process-ongoing'.