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Molecular Probes

To identify t...

Question

To identify the gene of interest in the gel matrix DNA __ are used.

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Solution

DNA probes are designed complementary to the known unique sequence comprising the gene of interest. Once the probes are added, they hybridize or attach only to the gene of interest and thus helps us identify the desired band in the gel matrix.

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Q. To identify the gene of interest in the gel matrix DNA __ are used.

Q. A gene of interest (GOI), amplified using PCR, can be isolated using agarose gel electrophoresis by ____i____ and the DNA is extracted out. This process is known as ____ii____.

Q. The given flow chart depicts the steps to transfer a desirable gene of interest into a plant.Isolation of desirable gene using restriction endonucleases and gel electrophoresis.
Regeneration of plants from the transformed cells to get transgenic plants.
Identify the missing steps (A, B and C) with regard to following statements and select the correct option.(i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule(ii) Selection of transformed cells(iii) Transferring the recombinant DNA molecules to the target cells

Q. Given below are the processes involved in recombinant DNA technology, which is used in the production of transgenic organisms:
1.Cutting of DNA at specific points to obtain the gene of interest
2.Isolation of DNA from the cellular components
3.Amplification of gene of interest
4.Inserting rDNA into the competent host cell
5.Inserting the gene of interest into a cloning vector
6.Separation of the isolated DNA fragments using agarose gel electrophoresis
From the options given below, choose the one which represents the correct order of these processes:

Q. is used for staining to visualise DNA in a gel.

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