To isolate protoplast, one needs:

chitinase.

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both pectinase and cellulase

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pectinase

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cellulase

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Solution

The correct option is B both pectinase and cellulase
Protoplast:

Protoplast is a plant cell with the cell wall removed.

Isolation of desired protoplasts:
Isolation of desired protoplasts is done by digesting the cell wall made up of pectin and cellulose with the help of digestive enzymes like pectinase, cellulase, and hemicellulase.

Final answer: c. both pectinase and cellulase.

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