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Introduction to Cell

To isolate pr...

Question

To isolate protoplast, one needs

A

Pectinase

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B

Cellulase

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C

Both pectinase and cellulase

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D

Chitinase

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Solution

The correct option is D Both pectinase and cellulase
Plant cell wall is arranged in layers and are composed of cellulose and soluble proteins along with pectin. Therefore, to isolate protoplast we need both pectinase and cellulase.
So, the correct option is 'Option C' .

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Similar questions

Q. Choose the correct option with respect to somatic hybridization.

(i) Protoplasts of different cells of the same plant are fused.

(ii) Protoplasts from cells of different species can be fused.

(iii) Cells are treated with cellulase and pectinase for protoplasm isolation.

Q. Given below are a few statements regarding somatic hybridisation. Choose the correct statements.
(i) Protoplasts of different cells of the same plant are fused.
(ii) Protoplasts from cells of different species can be fused.
(iii) Treatment of cells with cellulase and pectinase is mandatory
(iv) The hybrid protoplast contains characters of only one parental protoplast.

Q. Why is the enzyme cellulase used for isolating genetic material from plant cells but not for animal cells?

Q. The technique involving the isolation and fusion of protoplasts and culturing the hybrid protoplast to regenerate the whole plant is known as

Q. Which one of the following is the most convenient and popular source for isolation and culture of protoplasts?

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