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Question

To observe Amoeba and Paramecium in a water drop, the drop was taken on a glass slide. If both the organisms were clearly seen, then the stain used must be ________.

A
Methylene blue
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B
Iodine
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C
Safranin
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D
Eosin
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Solution

The correct option is A Methylene blue
Microorganisms can be observed under microscope using particular stain. To observe Amoeba and Paramecium in a water drop, the drop was taken on a glass slide. If both the organisms were clearly seen, then the stain used must be Methylene blue or thionin. Both these stains are LTP, that is Lipid transfer protein. They are used to observe animal cells like amoeba and paramecium through hybridization membranes like water. This stain makes the nucleus of amoeba and paramecium visible under microscope.
Thus, the correct answer is 'Methylene blue.'

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