Question

To prepare a temporary mount of a leaf peel for observing stomata, the chemicals used for staining and mounting respectively are

• A. safranin and iodine.
• B. safranin and glycerine.
• C. iodine and safranin.
• D. glycerine and iodine.

Answer 1

The correct option is B

safranin and glycerine.

The correct option is B.

The explanation for the correct option:

• Staining is the process of using a stain to color cells, tissues, components, or metabolic processes in a sample. Mounting entails affixing the samples to a glass microscope slide for analysis and observation.
• Safranin is used for staining, and glycerine is used for mounting.

The explanation for the incorrect options:

Option A:

• Safranin is used for staining, but iodine is not used for mounting.

Option C:

• Iodine is not used for staining, and safranin is not used for mounting.

Option D:

• Glycerine is not used for staining, and iodine is not used for mounting.

Therefore, the chemicals used for staining and mounting the leaf peel are safranin and glycerine, respectively.