1
Question
Tobacco plants resistant to nematodes have been developed by introduction of DNA that produces
Tobacco plants resistant to nematodes have been developed by introduction of DNA that produces
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Solution
The correct option is D Both sense and antisense RNA
RNA interference (RNAi) or silencing of RNA is a method used to prevent the expression of a gene.
Nematode parasites like the root-knot worm, Meloidogyne incognita, attack the root cells of plants (tobacco) which then form large galls or knots, damaging the crop and reducing the yield. Gene from M. incognita can be cloned in the Ti plasmid and can be introduced in the tobacco plant using the Agrobacterium vector.
It is done in such a way that both the sense and antisense RNA get expressed which are complementary to each other. This forms double stranded RNA. Cellular machinery processes the long double stranded RNA into shorter stretches.
These short stretches of dsRNA are taken up by the nematode while it feeds on the host plant parts and RNA interference (RNAi) is initiated. The particular gene(s) targeted (usually the gene(s) essential development) is silenced.
Therefore, the nematode cannot grow in the host plant and the plant gains pest resistance.
Hence, both sense and antisense RNA need to be produced to obtain double stranded RNA required for RNAi.
Hence the correct option is
a. Both sense and antisense RNA
RNA interference (RNAi) or silencing of RNA is a method used to prevent the expression of a gene.
Nematode parasites like the root-knot worm, Meloidogyne incognita, attack the root cells of plants (tobacco) which then form large galls or knots, damaging the crop and reducing the yield. Gene from M. incognita can be cloned in the Ti plasmid and can be introduced in the tobacco plant using the Agrobacterium vector.
It is done in such a way that both the sense and antisense RNA get expressed which are complementary to each other. This forms double stranded RNA. Cellular machinery processes the long double stranded RNA into shorter stretches.
These short stretches of dsRNA are taken up by the nematode while it feeds on the host plant parts and RNA interference (RNAi) is initiated. The particular gene(s) targeted (usually the gene(s) essential development) is silenced.
Therefore, the nematode cannot grow in the host plant and the plant gains pest resistance.
Hence, both sense and antisense RNA need to be produced to obtain double stranded RNA required for RNAi.
Hence the correct option is
a. Both sense and antisense RNA
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