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Question

Totipotency of the plant cells can be easily demonstrated by which of the following techniques?

A
DNA fingerprinting
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B
Tissue culture
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C
Multiple ovulation embryo transfer technology
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D
Biofortification
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Solution

The correct option is B Tissue culture
Totipotency of a cell is its ability to divide and differentiate into any cell type, such that it can develop into an entire organism.

In a mature plant, meristematic tissues actively divide and are essentially totipotent cells. But permanent tissues are those who have lost the ability to divide and have differentiated into a specific cell type.
In order to induce totipotency the permanent tissues must lose their function and start dividing (dedifferentiation).

Tissue culture is the technique of growing plant cells, tissues or organs in a sterile culture medium under controlled environmental conditions. In this technique certain plant parts, cell or tissue (explant) can bemade to develop into a whole plant by culturing it in a medium containing the appropriate nutrients and the right concentration of growth hormones.

In this technique the totipotency of cells to form the entire plant can be demonstrated.

DNA fingerprinting is the method of identifying DNA molecules of different individuals by locating differences in the arrangement of nucleotides in the specific repetitive regions of the DNA. As each individual has a unique DNA fingerprint, it helps in identifying criminals, confirming paternity testing, etc.

Biofortification is the technique of enhancing the nutritional value of a crop by selective plant breeding or genetic manipulation.

MOET or Multiple Ovulation Embryo Transfer Technology is an artificial technique for animal breeding.

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