Question

Transfer of DNA bands from an agrose gel to a nitrocellulose or nylon membrane is referred to as

• A. Western blotting
• B. Northern blotting
• C. Eastern blotting
• D. Southern blotting
  
• E. Gene transfer

Answer 1

The correct option is D Southern blotting

• Southern blotting combines the transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.
• Western is used for the separation and analysis of proteins.
• The Northern blotting is used for the separation and analysis of RNA.
• Gel electrophoresis is used for separation based on size or size and volume ratio.

So, the correct answer is option D.