Question

Transgenes are transferred to plants using Ti plasmid present in:

• A. Salmonella typhimurium
• B. Agrobacterium tumefaciens
• C. Bacillus thuringiensis
• D. Pseudomonas fluorescens

Answer 1

The correct option is B Agrobacterium tumefaciens

Ti plasmid or tumor inducing plasmid is isolated from the bacterium Agrobacterium tumefaciens and is used as a vector for transferring transgenes to plants. The gene of interest to be transferred is inserted into the Ti plasmid via genetic engineering methods. This plasmid is then inserted into Agrobacterium tumefaciens. The plant cells are then exposed to the modified Agrobacterium tumefaciens. The bacteria infects plant cells and the plasmid integrates with the plant genome thereby transferring thegene of interest to the plant.