Question

Transposon tagging has been effectively used for the gene cloning of

• A. Maize
• B. Fruitfly
• C. Man
• D. All of the above

Answer 1

The correct option is D All of the above

Transposon tagging refers to molecular isolation of transposable elements from genes in which the element resides. It is done by identification a mutant plant stock in which mutation is because of insertion of the transposable element into the gene, the creation of its genomic library followed by the screening of library with a clone for the transposable element. The selected clones have mutated gene lying adjacent to the element and are used to develop subclone containing gene sequences. This clone is then used to screen a genomic library containing DNA from a normal plant. Transposon tagging has been done in maize and fruit fly using class II transposons which are characterized by the presence of inverted repeats at their ends and ability to synthesize transposase. Class I transposons are abundant in the human genome which are tagged for gene cloning. The correct answer is D.