Let initial separation between the particles be l and this remains constant during the motion. If relative separation remains constant then relative velocity should be zero; hence relative acceleration of the particles should also be zero.
ar=a1−a2=0
or q1E−Fem1=Fe−q2Em2
or (q1m2+q2m1)Em1m2=Fe[1m1+1m2]
or (q1m2+q2m1)E=Fe(m1+m2)=14πε0q1q2l2(m1+m2)
After solving we get, l=12√q1q2(m1+m2)πε0E(q2m1+q1m2)