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Question

Two points separated by a distance of 0.1 mm can just be inspected in a microscope when light of wavelength 6000 ˙A is used. If the light of wavelength 4800 ˙A is used, then the limit of resolution will become

A
0.8 mm
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B
0.12 mm
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C
0.1 mm
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D
0.08 mm
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Solution

The correct option is D 0.08 mm
The limit of resolution of the microscope is given by,

dmin=1.22λ2μsinβ

Where, μrefractive index of the medium between object and objective.
βangle subtended by the radius of the objective with object

In the given situation, μ and sinβ are constant,

dminλ

d2d1=λ2λ1

The new limit of resolution

d2=λ2λ1×d1

d2=48006000×0.1 mm=0.08 mm

Hence, option (D) is correct.

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