Tyndall effect is observed only when:
(i) the diameter of the dispersed particles is not much smaller than the wavelength of the light used.
(ii) the refractive indices of dispersed phase and dispersion medium differ greatly in magnitude.
(iii) the size of the particles is generally between 10−11 and 10−9 m in diameter.
(iv) the dispersed phase and dispersion medium can be seen separately in the system.